Seeds of Amazonian Fabaceae as a source of new lectins

seeds from fifty native amazonian fabaceae species (representing subfamilies caesalpinioideae, mimosoideae and faboideae) were screened for the presence of new lectins. their crude protein extracts were assayed for hemagglutinating activity (ha). the protein fractions of anadenanthera peregrina, dimorphandra caudata, ormosia lignivalvis and swartzia laevicarpa exhibited ha, and this activity was inhibited by galactose or lactose but not by glucose or mannose. the crude extract of s. laevicarpa exhibited ha activity only after ion exchange chromatography, and its lectin was further purified by affinity chromatography on immobilized lactose. despite the large number of lectins that have been reported in leguminous plants, this is the first description of lectins in the genera anadenanthera, dimorphandra and ormosia. the study of lectins from these genera and from swartzia will contribute to the understanding of the evolutionary relationships of legume lectins in terms of their protein processing properties and structures.