Nuevo método para la cuantificación de la actividad endoglucanasa basado en el complejo celulosa-rojo congo

the aim of this work was to establish a method for quantifying endoglucanase activity based on the depolymerisation of the congo red-polysaccharide complex. the mathematical viscosity and absorbance patternsforcongo red-carboxymethylcellulose(cmc) culture media were thus described. the inoculated media'sviscosity and absorbance were monitored for 8 daysto measure the impact of a cellulolytic fungus such as trichodermaviride on the media. enzymeconcentration was calculated by correlating reducing sugars'absorbance test results using3,5-dinitrosalicylic acid (dns) with loss of cmc culture mediaabsorbance. four cellulolytic deuteromycetesstrains were testedto assess the technique'sapplicability;it was found that usingcmc media absorbancelosswas effective for differentiatingstrains'endoglucanase activity. the congored-cmc method was faster and more sensitive than the dns method. this new method should thusbe appliedin screening assays when bioprospecting cellulolytic fungi in soil.