Detección del Polimorfismo 1843 en el Gen Receptor de Ryanodina Mediante la Técnica de PCR-SSCP

pig stress syndrome (pss) is a genetic disease caused by a nucleotide mutation in the ryr-2 gene encoding the ryanodine receptor (ryr-1), considered an autosomal recessive condition. pss, or malignant hyperthermia, is characterised by the lowering of meat quality and animal death, leading to a pale, smooth and exhudative (pse) carcass. the syndrome is triggered in genetically-susceptible individuals either by anaesthetic agents, like halothane, or stress conditions, such as transport, crowding and mating. information about this syndrome's incidence and presence in colombia is currently quite scarce. this study was aimed at determining the presence of the ryr-i t-allele in a porcine population from the universidad national de colombia's marengo farm to identify individuals which were more susceptible to the syndrome. a total of 50 pigs (27 males and 23 females) were selected at random. cc genotype frequency for males was 0.59, 0.37 for the ct genotype and 0.04 for the tt genotype; in females this was 0.65 for the cc genotype, 0.13 for ct and 0.22 for tt. a total of 31 (62 %) individuals were considered healthy (cc), 13 (26 %) were carriers (ct) and six (12 %) were susceptible (tt). c and t allele frequency was 0.75 and 0.25, respectively. hardy-weinberg equilibrium comparison tests revealed that the population was in genetic disequilibrium (p ≤ 0.05). the results did show the presence of the allele responsible for the syndrome; this is a very significant factor to be considered when establishing an appropriate animal breeding programme by using marker-assisted selection.