Purificación de antígenos de Fasciola hepatica mediante electroelución y su aplicación inmunodiagnóstica mediante Western Blot en la infección animal

two polypeptides (14 kda and 29 kda) of adult fasciola hepatica were purified by electroelution and its diagnostic application through western blot was evaluated. the value of sensitivity was 95% and 97.5%, respectively and the value of specificity was 100% in both polypeptides. these antigenic components were efficient for the diagnosis of the prepatent stage of the infection