Quantifica？？o sérica de vitamina C por CLAE-UV e estudo de estabilidade

vitamin c, an exogenous antioxidant, is essential to human health. in this study, a method was validated to serum vitamin c quantification by hplc-uv. its stability with and without the use of tris [2-carboxy-ethyl] phosphine hydrochloride (tcep), at -20 and -80 °c, in serum and supernatant were also evaluated. analysis showed r2 > 0.99, precision cv% < 15% and % bias < 15%, being linear, precise and accurate. the stability test revealed that using tcep in serum storage at -20 and -80 °c or in supernatant at -80 °c the vitamin c levels remain stable for 30 and 12 days, respectively.