Correlación directa entre FEF25-75 y la actividad de la paraoxonasa plasmática en pacientes asmáticos adultos

introduction: plasma myeloperoxidase activity (mpo) (ec 1.11.1.7) has been related to several chronic-degenerative diseases such as asthma, which have in common a chronic inflammatory process. paraoxonase (pon-1) (ec 3.1.1.2), is an arylesterase enzyme that has a hydroperoxide catalytic function. this enzyme is a component of apo a-l located in hdl. pon1 is has been considered to protect lipoproteins, because it interrupts the oxidation process of ldl, conducive to the atherosclerotic process. objectives: to demonstrate that, a) oxidative stress in asthmatic patients correlates with the mpo activity, and b) demonstrate the role of pon 1 activity as a biomarker of their pulmonary function. methods: the activity of both enzymes was measured in plasma of patients with asthma and compared to a control group of healthy subjects. results: in the group of patients with asthma, mpo activity increased 42% (57.31 ± 7.2 u vs 33.34 ± 4.7 u p<0.05), while pon-1 activity decreased 52% (0.09 ±0.1 nmol p-nitrophenol/mg prot vs 0.05 ± 0.01 nmol p-nitrophenol/mg prot p<0.01). mpo activity showed an inverse correlation with fev, with a spearman r of -0.57, while pon-1 activiy showed a direct correlation with fef25-75, spearman r of0.64. conclusions: in this study we show, for the first time, that asthma patients, in whom there is a state of oxidative stress that affects the pulmonary function, pon-1 determination can be useful as a predictor of a better pulmonary function, whereas an increment in mpo activity could be associated to an acute inflammatory process, implicating cellular damage. * u = u/mg of protein'.