INFLUENCIA DE LA ADICIóN DE UNA FUENTE DE NITRóGENO EN LA PRODUCCIóN DE LIGNINASAS

large amounts of waste rich in lignin and hemicelullose named empty fruit brunch or “raquis” are produced during palm oil extraction process in colombia. these waste products were a good substrate for growing of pleurotus ostreatus in solid phase during 10 days of fermentation at 28°c. the aim of this study was to evaluate the enzymatic activity of laccase, manganese peroxidase and lignin peroxidase from the culture of pleurotus ostreatus on the empty fruit brunch of palm oil with different particle size (2, 3 and 4 cm) and nitrogen source (nh4cl and kno3). the activity of lignin peroxidase was not detected in the substrate without supplement. on the other hand, the better conditions for laccase activity (7,42 u/l) was in the substrate without supplement and with particle size of 3 cm, and for manganese peroxidase (20,29 u/l) was in the substrate supplemented with ammonium chloride (nh4cl) in carbon/nitrogen ratio of 20 and with particle size of 3 cm.