Nanotubo de carbono-chitosan en células HOS y THP-1

introduction: carbon nanotubes (cnt) are nanometer-sized structures used in medicine in the treatment of diseases, mainly in drug delivery in therapies against cancer. objectives: to study the internalization of carbon nanotubes modified with chitosan (cnt-ch) in human osteosarcom cells (hos) and human monocytes of acute leukemia (thp1). materials and methods: the cnts solubilized in chitosan 30% were characterized spectroscopically by uv-vis, fluorescence and raman. hos cells and thp-1 were treated with cnt-ch, the internalization was evaluated by giemsa staining with light microscopy, and cytotoxicity was determined using alamar blue assay. results: raman and fluorescence spectra showed the functionalization of the cnt with chitosan. after 24 h the ntc were internalized in the cell lines showing a cytoplasmic location and were not cytotoxic in any of both cell lines types. discussion: the properties of cnt-ch on cells allowing it to be potentially used as drug carrier salud uis 2011; 43(1): 21-26.