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Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)

DOI: 10.2478/v10120-009-0009-x

Keywords: CHD1, PCR, molecular sexing, blackbird, Turdus merula

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Abstract:

Turdus merula is one of most common bird species in Europe. This study verified a method for its sex identification by PCR with P2/P8 primers, based on the CHD1 gene polymorphism, proposed in earlier studies as a universal tool for sex identification in most bird species. Although there are some reports that PCR cannot determine sex in Eurasian blackbirds due to a lack of differences in intron lengths of CHD1-Z and CHD1-W genes, our study showed that such a possibility does exist, so it can contribute to an increased understanding of the biology of this species.

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