Production of (2R,3S)-2-Benzamidomethyl-3-Hydroxybutanoates by Immobilized Plant Cells of Parthenocissus Tricuspidata

Incubation of methyl 2-benzamidomethyl-3-oxobutanoate with cultured plant cells of Parthenocissus tricuspidata for 2 days afforded methyl (2R,3S)-2-benzamidomethyl-3-hydroxybutanoate with 100% de and 99% ee in 51% yield. The use of immobilized cells of P. tricuspidata in calcium alginate gel improved the yield of the product (95% yield) with 100% de and 99% ee. The immobilized cells of P. tricuspidata maintained the high potential for the reduction of methyl 2-benzamidomethyl-3-oxobutanoate (85% yield) after 5 times of usage.