Genetic Relationships among 17 Bramble Cultivars and 11 Wild Excellent Rubus Germplasms from China Revealed by RAPD

Genetic relationships among 17 bramble cultivars introduced abroad and 11 wild excellent Rubus germplasms from China were analyzed by RAPD markers. A total of 500 DNA bands were amplified by 22 primers and 490 out of 500 bands (98%) were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 22.28. All materials could be distinguished by RAPD markers with 0.687-0.964 genetic similarity coefficients. According to the data, a dendrogram of genetic relationship, which was constructed using the UPGMA method, showed that all the tested cultivars and wild species (varieties) were classified into four groups. The present results verify that the close genetic relationship between ten raspberry cultivars and seven excellent wild germplasms from section (subgenus) Idaeobatus of the genus Rubus, while a little far genetic relationship between ten raspberry cultivars and seven blackberry cultivars. There was a far genetic relationship between seven wild germplasms from section (subgenus) Idaeobatus and four wild germplasms from section (subgenus) Malachobatus. The two cultivated bramble groups (raspberry and blackberry) could be completely distinguished, but it was failed to distinguish red raspberry with even red raspberry in raspberry group.