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世界科学技术-中医药现代化 2012
Study on Protein Detection Method in Qingkailing Injection
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Abstract:
This study was aimed to establish a method for determining protein content in Qingkailing Injection. Biuret colorimetry was used to determine the protein which had been precipitated by trichloroacetic acid. SDSpolyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the molecular weight of the protein. The results showed that molecular weight of protein in Qingkailing Injection is between 3 KD to 7 KD. It was concluded that SDS-PAGE can be used to determine the protein in Qingkailing Injection. This method is simple and rapid.