Analysis of factors influencing the infectivity of HIV-1 pseudotyped virus during packaging by cell line
HIV-1假病毒包装的重要影响因素分析

Objective] A serials of factors which may have influence on producing the HIV-1 pseudotyped virus are analysed to establish an optimal way to generate this type of virus. Methods] A set of factors such as the transfection reagents, the ratios of plasmid and transfection, the serum in the media and cell cycle is being analysis to find the optimal condition. Results] The optimal N/P is range from 8 to 40 , the serum can influence the stability of pseudotype virus and in the stage S and G2/M, transfection efficiency is high than the stage G0/G1. Conclusion] High titers of pseudotyped HIV-1 virus can be obtained by means of using transfection reagents PEI and through combinatorial optimization of PEI, plasmid amount and FBS type.