Cloning of royal jelly AccMRJP1 gene from Apis cerana cerana and its expression in Escherichia coli
中华蜜蜂AccMRJP1基因克隆及在大肠杆菌中的表达

Three clones of AccMRJP1 were screened out from the sequenced brain cDNA library of Chinese honeybee according to the expression sequence tag(EST) of AccMRJP1.Through identifying with polymerase chain reaction(PCR) and sequencing,a AccMRJP1 clone containing an open reading frame(ORF) of 1302 nucleotides encoding a protein of 433 amino acids was determined.The AccMRJP1 had 99.8% similarity with the previously reported AccMRJP1 sequence(AY279539) in amino acid sequences.The AccMRJP1 was sub-cloned into the pr...