Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish, Siniperca chuatsi
鳜肌酸激酶M-CK cDNA的克隆与组织表达分析

The creatine kinase(CK) cDNA from the mandarin fish Siniperca chuatsi was cloned by RT-PCR and rapid amplification of cDNA ends (RACE) methods.The structural characteristics and phylogeny of this gene were analyzed.Sequence analysis revealed a 1586 bp cDNA sequence containing 92 bp 5'-untranslated region,348 bp 3'-untranslated region and 1146 bp open reading frame (ORF),which encoded 381 amino acids.Conserved sequence blocks of vertebrate CKs and diagnostic boxes for the muscle CK(M-CK) isozyme were identif...