Influences of growth regulators on callus induction, ephedrine and pseudoephedrine contents and chemical analysis of mature embryo of Ephedra strobilacea Bunge

An experiment was carried out to develop a protocol for callus induction of Ephedrastrobilacea Bunge from embryo. Different types and concentrations of plant growth regulators weretested in order to obtain the best callus formation, and the following hormonal ranges: 2, 4-D: 1 Kin: 1(mg L-1) and 2, 4-D: 1 BA: 0.1 (mg L-1) had showed the highest callus induction and the highest freshweight, respectively. Meanwhile, ephedrine and pseudo-ephedrine contents in callus were analyzed andwe found that at NAA: 1 Kin: 1 (mg L-1) range the ephedrine and pseudo-ephedrine contents hadsignificantly increased compared to the other treatments.