Horse gram [ Macrotyloma uniflorum (Lam.) Verdc.] seeds containing high concentrations of fatty acids, flavonols and minerals should provide government, public and private organizations with a nutritious and healthy food for use by malnourished and food deprived people worldwide. Seeds from seven horse gram accessions, geographically adapted to Griffin, GA, USA were analyzed for fatty acid, flavonol, and mineral concentrations using gas chromatography, reverse-phase high performance liquid chromatography, and inductively coupled plasma-optical emission spectroscopy, respectively. Significant year effects occurred for stearic, oleic, linoleic, arachidic, gadoleic, and lignoceric acids. Oleic, linoleic, and linolenic acid ranged from 8.9%–16.8%, 40.3%–45.6%, and 11.6%–14.3%, respectively, as percent of total fatty acids measured (total oil ranged from 2.32% to 2.87%). Seed concentrations of myricetin, quercetin, and kaempferol ranged from 0–36 μg/g DW, 0–27 μg/g DW, and 240–316 μg/g DW, respectively and the only year effect was observed for kaempferol among the horse gram accessions. Year effects were found for Fe, K, Mg, Mn, Ni, and S. Mean concentrations of macrominerals (Ca, K, Mg, P, and S) and microminerals (Cu, Fe, Mn, Ni, and Zn) ranged from 1.3–14 mg/g DW, and 1.0–95.0 μg/g DW, respectively. Several correlations were observed among several fatty acids, flavonols, and minerals. The mono-unsaturated fatty acid, oleic acid correlated significantly with linoleic acid ( r = ？0.64), arachidic acid ( r = ？0.61), Ca ( r = 0.50) and Zn ( r = 0.51, all at P < 0.01). The flavonol, myricetin correlated significantly with quercetin ( r = 0.92, P < 0.0001), while quercetin correlated with Ca ( r = 0.82, P < 0.0001) and kaempferol correlated with Mg ( r = 0.61, P < 0.01). Several mineral correlations were found including Fe with K ( r = 0.66) and Mg ( r = 0.56, both at P < 0.01). These seven horse gram accessions can be used in breeding programs to facilitate the production of superior cultivars with favorable fatty acid profiles, flavonol content, and mineral compositions.
References
[1]
Youdim, K.A.; Martin, A.; Joseph, J.A. Essential fatty acids and the brain: Possible health implications. Int. J. Dev. Neurosci. 2000, 18, 383–399, doi:10.1016/S0736-5748(00)00013-7.
[2]
Brielmann, H.L.; Setzer, W.N.; Kaufman, P.B.; Kirakosyan, A.; Cseke, L.J. Natural Products from Plants, 2nd; Cseke, L.J., Kirakosyan, A., Kaufman, P.B., Warber, S.L., Duke, J.A., Brielmann, H.L., Eds.; Taylor and Francis Group: Boca Raton, FL, USA, 2006; pp. 1–49.
[3]
Kang, N.J.; Jung, S.K.; Lee, K.W.; Lee, H.J. Myricetin is a potent chemopreventive phytochemical in skin carcinogenesis. Ann. N.Y. Acad. Sci. 2011, 1, 124–132.
[4]
Sun, F.; Zheng, X.Y.; Ye, J.; Wu, T.T.; Wang, J.I.; Chen, W. Potential anticancer activity of myricetin in human T24 bladder cancer cells both in vitro and in vivo. Nutr. Cancer 2012, 64, 599–606, doi:10.1080/01635581.2012.665564.
[5]
Phillips, P.A.; Sangwan, V.; Borja-Cacho, D.; Dudeja, V.; Vickers, S.M.; Saluja, A.K. Myricetin induces pancreatic cancer cell death via the induction of apoptosis and inhibition of the phosphatidylinositol 3-kinase (P13K) signaling pathway. Cancer Lett. 2011, 308, 181–188, doi:10.1016/j.canlet.2011.05.002.
[6]
Yu, M.S.; Lee, J.; Lee, J.M.; Kim, Y.; Chin, Y.W.; Jee, J.G.; Keum, Y.S.; Jeong, Y.J. Identification of myricetin and scutellarein as novel chemical inhibitors of the SARS coronavirus helicase, nsP13. Bioorg. Med. Chem. Lett. 2012, 22, 4049–4054, doi:10.1016/j.bmcl.2012.04.081.
[7]
Edwards, R.L.; Lyon, T.; Litwin, S.E.; Rabovsky, A.; Symons, J.D.; Jalili, T. Quercetin reduces blood pressure in hypertensive subjects. J. Nutr. 2007, 137, 2405–2411.
[8]
Loke, W.M.; Hodgson, J.M.; Proudfoot, J.M.; McKinley, A.J.; Puddey, I.B.; Croft, K.D. Pure dietary flavonoids quercetin and (-)-epicatechin augment nitric oxide products and reduce endothelin-1 acutely in healthy men. Am. J. Clin. Nutr. 2008, 88, 1018–1025.
[9]
Davis, J.M.; Carlstedt, C.J.; Chen, S.; Carmichael, M.D.; Murphy, E.A. The dietary flavonoid quercetin increases VO(2max) and endurance capacity. Int. J. Sport Nutr. Exerc. Metab. 2010, 20, 56–62.
[10]
Kanzaki, N.; Saito, K.; Maeda, A.; Kitagawa, Y.; Kiso, Y.; Watanabe, K.; Tomonaga, A.; Nagaoka, I.; Yamaguchi, H. Effect of a dietary supplement containing glucosamine hydrochloride, chondroitin sulfate and quercetin glycosides on symptomatic knee osteoarthiritis: A randomized, double-blind, placebo-controlled study. J. Sci. Food Agric. 2012, 92, 862–869, doi:10.1002/jsfa.4660.
[11]
Bouis, H.E.; Welch, R.M. Biofortification—A sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop Sci. 2010, 50, S20–S32.
[12]
Keatinge, J.D.H.; Waliyar, F.; Jamnadas, R.H.; Moustafa, A.; Andrade, M.; Drechsel, P.; Hughes, J.d’A.; Kadirvel, P.; Luther, K. Relearning old lessons for the future of food—By bread alone no longer: Diversifying diets with fruit and vegetables. Crop Sci. 2010, 50, S51–S62, doi:10.2135/cropsci2008.12.0695.
[13]
Kadam, S.S.; Salunkhe, D.K. Nutritional composition, processing, and utilization of horse gram and moth bean. Crit. Rev. Food Sci. Nutr. 1985, 22, 1–26.
[14]
Bravo, L.; Siddhuraju, P.; Saura-Calixto, F. Composition of underexploited Indian pulses. Comparison with common legumes. Food Chem. 1999, 64, 185–192, doi:10.1016/S0308-8146(98)00140-X.
[15]
Shobana, G.; Sivaelango, G.; Kumaravel, P. Evaluation of antihyperlipidaemic effect of macrotyloma uniflorum seed extract in experimental hyperlipidaemia. Res. J. Pharm. Technol. 2012, 5, 353–356.
[16]
Gupta, L.H.; Badole, S.L.; Bodhankar, S.L.; Sabharwal, S.G. Antidiabetic potential of α-amylase inhibitor from the seeds of Macrotyloma uniflorum in streptozotocin-nicotinamide-induced diabetic mice. Pharm. Biol. 2011, 49, 182–189, doi:10.3109/13880209.2010.507633.
[17]
Kawsar, S.M.A.; Uddin, M.S.; Huq, E.; Nahar, N.; Ozeki, Y. Biological investigation of Macrotyloma uniflorum Linn. extracts against some pathogens. J. Biol. Sci. 2008, 8, 1051–1056, doi:10.3923/jbs.2008.1051.1056.
[18]
Joshi, U.N.; Luthra, Y.P. Fatty acid profile of horse gram seeds. In Advances in Arid Legumes Research, Proceedings of the National Symposium on arid legumes, for food nutrition security and promotion of trade, Hisar, India, 15–16 May 2002.
[19]
Krishna, A.G.G.; Prabhakar, J.V.; Aitzetmuller, K. Tocopherol and fatty acid composition of some Indian pulses. J. Am. Oil Chem. Soc. 1997, 74, 1603–1606, doi:10.1007/s11746-997-0084-2.
[20]
Sreerama, Y.N.; Sashikala, V.B.; Pratape, V.M. Variability in the distribution of phenolic compounds in milled fractions of chickpea and horse gram: Evaluation of their antioxidant properties. J. Agric. Food Chem. 2010, 58, 8322–8330, doi:10.1021/jf101335r.
[21]
Diaz-Batalla, L.; Widholm, J.M.; Fayey, G.C., Jr.; Castano-Tostado, E.; Paredes-Lopez, O. Chemical components with health implications in wild and cultivated Mexican common bean seeds (Phaseolus vulgaris L.). J. Agric. Food Chem. 2006, 54, 2045–2052, doi:10.1021/jf051706l.
