CYTOGENETIC STUDIES OF CELL LINES; MDCK AND VERO

The established cell lines; MDCK and Vero were studied to find out transformation of chromosome number. The various techniques like G-Banding and C-banding were also studied. The changes in chromosome number were noticed which could be related to number of passages given to cell lines. Present studies observed 76 chromosomes minimum and 81 maximum in MDCK cells, whereas 54 chromosomes minimum and 61 maximum in case of Vero cells. Hence the inconsistency in chromosome number implies that the MDCK cells have been moderately transformed, which could be because of 15 and 21 passages respectively.