Expression of CXC Chemokine, IP-10/Mob-1 is Regulated by Specific p38,CAMK-II, NF oB and Staurosporine Regulatory Pathways in Primary Hepatocytes

Hepatocytes were isolated from male Sprague Dawley rat liver and cultured on collagen Type-I matrix. We employed western blotting analysis to investigate the expression of a CXC chemokine IP-10/Mob-1 by isolated and cultured hepatocytes in the presence of inhibitors of specific protein kinases. The expression of the CXC chemokines IP-10/Mob-1 was inhibited in the presence of inhibitors of specific protein kinases and signal pathways SB203580, MG132, KN62 and Staurosporine. Hence, these in vitro data may aid to a better understanding of the pathways in chronic liver injuries and identify clinical studies that may aid in treatment or prevention of these conditions. Furthermore, there may be further potential to prevent changes to hepatocyte phenotype and allow isolation of hepatocytes with a greater physiological phenotype.