Development of a Simple Method for a New Immunoconjugate Utilizing Laccase

Enzyme immunoassays utilize immunoglobulins conjugated to different enzyme labels or marker enzymes. In the current study, an immunoconjugate is being developed utilising the fungal enzyme laccase. The efficacy of this newly developed immunolaccase conjugate, prepared by standardized conjugation methods (glutaraldehyde and periodate based), has been demonstrated in indirect enzyme-immunoassay, for detection of purified rabbit IgG. The results of the assay indicated this newly developed immunolaccase conjugate to be potent for the detection of purified rabbit IgG when compared with commercially available peroxidase conjugated immunoglobulin or immunoperoxidase conjugate.