Histological study for improvement of the sciatic nerve injury of the rat by administration of oil of Nigella Sativa

Twenty five adult rats of about 200 gm weight were subjected to the left sciatic nerve injury by micro artery forceps for 10 second. After nerve injury, the animals were classified into five groups A, B, C, D and E, each containing five animals. All the animals were administered the oil of Nigella sativa in a dose of 200 μml. The schedule of administration was such that group A was dosed for one week, group B for two weeks , group C for one month and group D for two months successively, while group E remained as control. At the end of experimental period, the animals were sacrificed and approximately 5 mm segments of the sciatic nerves was obtained from each animal, processed for histological technique to demonstrate the effect of the oil Nigella sativa on the architecture of the sciatic nerve. The present results demonstrated the efficacy of the oil improved the regeneration of the nerve injury better and faster than that of the control animals and this could be interpreted as the effect of oil of Nigella sativa on the mediators of inflammation and their cytoprotective and antioxidant actions.