Evaluation of Potential Redox Modulatory and Chemotherapeutic Effects of a Proprietary Bioactive Silicate Alka-Vita /Alka-V6 /Alkahydroxy (AVAH)

Summary: A proprietary modified sodium silicate manufactured by Cisne enterprises Inc. (Odessa, TX) was evaluated for its ability to modulate various parameters relevant to establishment and progression of cancer. Antimutagenic effects were determined using Ames test. Prevention of colon cancer cell (HT-29) attachment and growth was done using standard methods. Apoptotic induction was measured by DNA fragmentation (DNAF) assay. Malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activity were measured using standard assays. Chemical structure determined by nuclear magnetic resonance (NMR) and infra-red (IR) spectroscopy suggested that the product was a mixture of trimeric sodium silicate and sodium silicate pentahydrate. A dose-dependent reduction in attachment (IC50 = 0.15 mM) and growth (IC50 = 0.18 mM) of HT-29 was observed. At low levels (0.029-2.9 mM) the product was able to prevent various sodium azide induced mutations in Ames test. A dose dependent increase in DNAF suggested induction of apoptosis. A drop in MDA levels and increased in GHS, SOD and CAT activities suggested induction of antioxidant response. We conclude that the product may have cancer chemotherapeutic properties in vitro due to its unique structural and electrochemical properties. In vivo tests are imperative to determine true effectiveness. Industrial relevance: Silicates are extensively used in cosmetic, food and environmental industries. They are also useful in agriculture as anti-fungal agents and have growth promoting functions. Significance of silicates in human health is not very well understood. It has been classified as a trace mineral and may play a role in bone and joint development. Empirical evidence suggests that certain forms of silicates may have therapeutic potential against chronic and infectious diseases. Understanding their health promoting properties and elucidating their mechanism of action may result in development of new strategies for managing these diseases.