Dissection of Cerebral White Matter. Importance for Neurosurgical Training.

The understanding of the three-dimensional architecture of the brain is a fundamental condition to the anatomist and the neurosurgeon, especially during their training in basic or surgical neuroanatomy.Demonstrate through the Klingler’s technique the anatomical three-dimensional disposition of white matter tracts.According to the Klingler’s method (Atlas Cerebri Humani - The inner structure of the brain), is preconized that the material should be fresh. A solution of commercial formalin (5%) and distilled water (95%) should be prepared; the material should be immersed and after the course of 4 weeks immersed in the fixing solution, it should be placed on a tray in the freezer (-10°C) during 8 days. Once removed from the freezer, the material must remain in a formalin 3% solution.We worked with 10 brains prepared as the previously described technique. Our main instruments are: delicate type dissecting Adson forceps without teeth and watchmaker’s forceps, and wooden spatulas with a diameter of 2 and 4 mm.We obtained pieces for the demonstration of various tracts that they are located from the cortex to the depth with a description from the external, medial and basal cerebral aspect.While the dissection of white matter is not a new technique that is used for the study and understanding of the brain, remains extremely useful. The training in this anatomical analysis technique has an essential importance for each neurosurgeon.