Investigation of Some the Activities of G6PDH and LDH in the Liver of Adult Male Wistar Rats Following the Administration of Aqueous Extract of Ocimum sanctum

The aim of this study was to investigate some of the effects of aqueous extract of Ocimum sanctum on glucose-6-phosphate dehydrogenase and lactate dehydrogenase in adult male wistar rats (Rattus norvergicus). Thirty two adult male wistar rats weighing between 150-230 g used in this experiment were divided into four groups of eight (n = 8) animals each. Each animal in Group I, II, III and control were administered respectively with 250, 150, 50 mg/kg bw of the extract per day for a period of 3 weeks (21 days) through the oral route while the last group was administered phosphate buffered saline as the control. At the end of this investigation, a dose-dependent increased activity of the enzymes was observed. This observation affirms the earlier observed hepato-protective effect of Ocimum sanctum. This experiment also suggests that carbohydrates are facilitated more along the hexose monophosphate pathway for G6PDH and the generation of lactate may serve as substrate for gluconeogenesis.