Reverse Phase Ultra Fast Liquid Chromatographic Method for Determination of Gemifloxacin Mesylate in Tablet Dosage Form

A novel, accurate and precise reverse phase ultra fast liquid chromatographic method for determination of gemifloxacin mesylate has been developed and validated. Separation was achieved on an Enable C18G column (250mm × 4.6mm i.d.,5μm) using methanol: 10mM TBAHS (70:30, v/v) as mobile phase at a flow rate of 1.0ml/min and PDA detection at 271nm. Linearity was observed in the concentration range of 1.0-200 μg/ml(r2=1). The method was validated for accuracy, precision, stability, specificity, robustness and system suitability. Forced degradation was performed by using HCl, NaOH, H2O2 , thermal and UV radiation. The method was used successfully for the determination of gemifloxacin 2 2 mesylate in tablet dosage form.