Bioethanol as a fossil fuel additive to decrease environmental pollution and reduce the stress of the decline in crude oil availability is becoming increasingly popular. This study aimed to evaluate the concentration of bioethanol obtainable from fermenting cashew apple juice by the microorganism Saccharomyces cerevisiae Y2084 and Vin13. The fermentation conditions were as follows: initial sugar = 100？g/L, pH = 4.50, agitation = 150？rpm, temperatures = 30°C (Y2084) and 20°C (Vin13), oxygen saturation = 0% or 50%, and yeast inoculum concentration = ~8.00？Log？CFU/mL. The maximum ethanol concentration achieved by Y2084 was 65.00？g/L. At 50% oxygen the fermentation time was 5 days, whilst at 0% oxygen the fermentation time was 11 days for Y2084. The maximum ethanol concentration achieved by Vin13 was 68.00？g/L. This concentration was obtained at 50% oxygen, and the fermentation time was 2 days. At 0% oxygen, Vin13 produced 31.00？g/L of ethanol within 2 days. Both yeast strains produced a higher glycerol concentration at 0% oxygen. Yeast viability counts showed a decrease at 0% oxygen and an increase at 50% oxygen of both yeast stains. Other analyses included measurement of carbon dioxide and oxygen gases, process monitoring of the fermentation conditions, and total organic carbon. Gas analysis showed that carbon dioxide increased in conjunction with ethanol production and oxygen decreased. Process monitoring depicted changes and stability of fermentation parameters during the process. Total organic carbon analysis revealed that aerobic fermentation (50% oxygen) was a more efficient process as a higher carbon recovery (95%) was achieved. 1. Introduction The twenty-first century is plagued by difficulties like the decrease in resources of fossil fuel, rapid rise in greenhouse gas emissions contributing to global warming, and the lack of capability to meet the increasing energy demands. In trying to reduce the impact of these global concerns, bioethanol produced from renewable resources like biomass components has created significant interest. Bioethanol is a biofuel produced from biomass via biochemical procedures [1]. In general, bioethanol production is a three-stage process of: (i) hydrolysis, (ii) fermentation and (iii) distillation [2]. During hydrolysis, starch is converted from biomass feedstocks (cereal grains, lignocellulose, and macroalgae) into fermentable monosaccharide sugars [1–3]. Fermentation is a process which involves a sugar-rich substrate and a microorganism (MO) to bring about a chemical change in a closed aerobic or anaerobic atmosphere.
References
[1]
A. Demirbas, “Biofuels sources, biofuel policy, biofuel economy and global biofuel projections,” Energy Conversion and Management, vol. 49, no. 8, pp. 2106–2116, 2008.
[2]
K. R. Jegannathan, E. S. Chan, and P. Ravindra, “Harnessing biofuels: a global Renaissance in energy production?” Renewable and Sustainable Energy Reviews, vol. 13, no. 8, pp. 2163–2168, 2009.
[3]
C. S. Goh and K. T. Lee, “A visionary and conceptual macroalgae-based third-generation bioethanol (TGB) biorefinery in Sabah, Malaysia as an underlay for renewable and sustainable development,” Renewable and Sustainable Energy Reviews, vol. 14, no. 2, pp. 842–848, 2010.
[4]
ó. J. Sánchez and C. A. Cardona, “Trends in biotechnological production of fuel ethanol from different feedstocks,” Bioresource Technology, vol. 99, no. 13, pp. 5270–5295, 2008.
[5]
B. Amigun, R. Sigamoney, and H. von Blottnitz, “Commercialisation of biofuel industry in Africa: a review,” Renewable and Sustainable Energy Reviews, vol. 12, no. 3, pp. 690–711, 2008.
[6]
E. W. de Menezes, R. Catalu？a, D. Samios, and R. D. Silva, “Addition of an azeotropic ETBE/ethanol mixture in eurosuper-type gasolines,” Fuel, vol. 85, no. 17-18, pp. 2567–2577, 2006.
[7]
D. Turner, H. Xu, R. F. Cracknell, V. Natarajan, and X. Chen, “Combustion performance of bio-ethanol at various blend ratios in a gasoline direct injection engine,” Fuel, vol. 90, no. 5, pp. 1999–2006, 2011.
[8]
G. M. Walker, “Fuel ethanol: current production and future challenges,” Journal of the Institute of Brewing, vol. 117, no. 1, pp. 3–22, 2011.
[9]
S. Kim and B. E. Dale, “Global potential bioethanol production from wasted crops and crop residues,” Biomass and Bioenergy, vol. 26, no. 4, pp. 361–375, 2004.
[10]
M. Linde, M. Galbe, and G. Zacchi, “Bioethanol production from non-starch carbohydrate residues in process streams from a dry-mill ethanol plant,” Bioresource Technology, vol. 99, no. 14, pp. 6505–6511, 2008.
[11]
M. Balat and H. Balat, “Recent trends in global production and utilization of bio-ethanol fuel,” Applied Energy, vol. 86, no. 11, pp. 2273–2282, 2009.
[12]
E. D. Deenanath, S. Iyuke, and K. Rumbold, “The bioethanol industry in Sub-Saharan Africa: history, challenges, and prospects,” Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 416491, 11 pages, 2012.
[13]
M. Linde, M. Galbe, and G. Zacchi, “Steam pretreatment of acid-sprayed and acid-soaked barley straw for production of ethanol,” Applied Biochemistry and Biotechnology, vol. 130, no. 1–3, pp. 546–562, 2006.
[14]
M. Linde, M. Galbe, and G. Zacchi, “Simultaneous saccharification and fermentation of steam-pretreated barley straw at low enzyme loadings and low yeast concentration,” Enzyme and Microbial Technology, vol. 40, no. 5, pp. 1100–1107, 2007.
[15]
G. Najafi, B. Ghobadian, T. Tavakoli, and T. Yusaf, “Potential of bioethanol production from agricultural wastes in Iran,” Renewable and Sustainable Energy Reviews, vol. 13, no. 6-7, pp. 1418–1427, 2009.
[16]
Y. Sun and J. Cheng, “Hydrolysis of lignocellulosic material for ethanol production: a review,” Bioresource Technology, vol. 83, no. 1, pp. 1–11, 2002.
[17]
T. L. Honorato, M. C. Rabelo, L. R. B. Gon？alves, G. A. S. Pinto, and S. Rodrigues, “Fermentation of cashew apple juice to produce high added value products,” World Journal of Microbiology and Biotechnology, vol. 23, no. 10, pp. 1409–1415, 2007.
[18]
D. A. Luz, A. K. O. Rodrigues, F. R. C. Silva et al., “Adsorptive separation of fructose and glucose from an agroindustrial waste of cashew industry,” Bioresource Technology, vol. 99, no. 7, pp. 2455–2465, 2008.
[19]
M. S. Silveira, C. P. M. L. Fontes, A. A. Guilherme, F. A. N. Fernandes, and S. Rodrigues, “Cashew apple juice as substrate for lactic acid production,” Food and Bioprocess Technology, vol. 5, no. 3, pp. 947–953, 2012.
[20]
A. J. MacLeod and N. G. de Troconis, “Volatile flavour components of cashew “apple” (Anacardium occidentale),” Phytochemistry, vol. 21, no. 10, pp. 2527–2530, 1982.
[21]
M. V. P. Rocha, A. H. S. Oliveira, M. C. M. Souza, and L. R. B. Gon？alves, “Natural cashew apple juice as fermentation medium for biosurfactant production by Acinetobacter calcoaceticus,” World Journal of Microbiology and Biotechnology, vol. 22, no. 12, pp. 1295–1299, 2006.
[22]
A. M. Pacheco, D. R. Gondim, and L. R. B. Goncalves, “Ethanol production by fermentation using immobilized yeast cells,” Applied Biochemistry and Biotechnology, vol. 161, no. 1–8, pp. 209–217, 2010.
[23]
A. Osho, “Ethanol and sugar tolerance of wine yeasts isolated from fermenting cashew apple juice,” African Journal of Biotechnology, vol. 4, no. 7, pp. 660–662, 2005.
[24]
C. D. Rakopoulos, K. A. Antonopoulos, D. C. Rakopoulos, D. T. Hountalas, and E. G. Giakoumis, “Comparative performance and emissions study of a direct injection Diesel engine using blends of Diesel fuel with vegetable oils or bio-diesels of various origins,” Energy Conversion and Management, vol. 47, no. 18-19, pp. 3272–3287, 2006.
[25]
T. L. Honorato and S. Rodrigues, “Dextransucrase stability in cashew apple juice,” Food and Bioprocess Technology, vol. 3, no. 1, pp. 105–110, 2010.
[26]
T. O. Akinwale, “Cashew apple juice: its use in fortifying the nutritional quality of some tropical fruits,” The European Food Research and Technology, vol. 211, no. 3, pp. 205–207, 2000.
[27]
M. T. S. Trevisan, B. Pfundstein, R. Haubner et al., “Characterization of alkyl phenols in cashew (Anacardium occidentale) products and assay of their antioxidant capacity,” Food and Chemical Toxicology, vol. 44, no. 2, pp. 188–197, 2006.
[28]
C. M. A. Chagas, T. L. Honorato, G. A. S. Pinto, G. A. Maia, and S. Rodrigues, “Dextransucrase production using cashew apple juice as substrate: effect of phosphate and yeast extract addition,” Bioprocess and Biosystems Engineering, vol. 30, no. 3, pp. 207–215, 2007.
[29]
M. C. Rabelo, C. P. M. L. Fontes, and S. Rodrigues, “Enzyme synthesis of oligosaccharides using cashew apple juice as substrate,” Bioresource Technology, vol. 100, no. 23, pp. 5574–5580, 2009.
[30]
D. C. P. Campos, A. S. Santos, D. B. Wolkoff, V. M. Matta, L. M. C. Cabral, and S. Couri, “Cashew apple juice stabilization by microfiltration,” Desalination, vol. 148, no. 1–3, pp. 61–65, 2002.
[31]
A. D. T. Pinheiro, M. V. P. Rocha, G. R. MacEdo, and L. R. B. Gon？alves, “Evaluation of cashew apple juice for the production of fuel ethanol,” Applied Biochemistry and Biotechnology, vol. 148, no. 1–3, pp. 227–234, 2008.
[32]
E. D. Deenanath, S. E. Iyuke, and D. Lindsay, “Enzymatic hydrolysis of bitter sorghum for bioethanol production,” Master Brewers Association of the Americas-MBAA TQ, 2010.
[33]
T. L. Pattison, I. Geornaras, and A. von Holy, “Microbial populations associated with commercially produced South African sorghum beer as determined by conventional and Petrifilm？ plating,” International Journal of Food Microbiology, vol. 43, no. 1-2, pp. 115–122, 1998.
[34]
T. M. Lapara, J. E. Alleman, and P. G. Pope, “Miniaturized closed reflux, colorimetric method for the determination of chemical oxygen demand,” Waste Management, vol. 20, no. 4, pp. 295–298, 2000.
[35]
B. L. Attri, “Effect of initial sugar concentration on the physic-chemical characteristics and sensory qualities of cashew apple wine,” Natural Product Radiance, vol. 8, no. 4, pp. 374–379, 2009.
[36]
S. Boyes, P. Strübi, and H. Dawes, “Measurement of protein content in fruit juices, wine and plant extracts in the presence of endogenous organic compounds,” LWT—Food Science and Technology, vol. 30, no. 8, pp. 778–785, 1997.
[37]
A. J. A. van Maris, A. A. Winkler, M. Kuyper, W. T. A. M. de Laat, J. P. van Dijken, and J. T. Pronk, “Development of efficient xylose fermentation in saccharomyces cerevisiae: xylose isomerase as a key component,” Advances in Biochemical Engineering/Biotechnology, vol. 108, pp. 179–204, 2007.
[38]
W. H. van Zyl, L. R. Lynd, R. den Haan, and J. E. McBride, “Consolidated bioprocessing for bioethanol production using saccharomyces cerevisiae,” Advances in Biochemical Engineering/Biotechnology, vol. 108, pp. 205–235, 2007.
[39]
T. Neelakandan and G. Usharani, “Optimization and production of bioethanol from cashew apple juice using immobilized yeast cells by Saccharomyces cerevisiae,” America-Eurasian Journal of Scientific Research, vol. 4, no. 2, pp. 85–88, 2009.
[40]
M. P. Brosnan, D. Donnelly, T. C. James, and U. Bond, “The stress response is repressed during fermentation in brewery strains of yeast,” Journal of Applied Microbiology, vol. 88, no. 5, pp. 746–755, 2000.
[41]
S. Gutt and G. Gutt, “Factors influencing the fermentation process and ethanol yield,” Romanian Biotechnological Letters, vol. 14, no. 5, pp. 4648–4657, 2009.
[42]
S. Kura, H. Nishiumi, and Y. Kawase, “Oxygen transfer in a stirred loop fermentor with dilute polymer solutions,” Bioprocess Engineering, vol. 8, no. 5-6, pp. 223–228, 1993.
[43]
C. Fornairon-Bonnefond, E. Aguera, C. Deytieux, J. M. Sablayrolles, and J. M. Salmon, “Impact of oxygen addition during enological fermentation on sterol contents in yeast lees and their reactivity towards oxygen,” Journal of Bioscience and Bioengineering, vol. 95, no. 5, pp. 496–503, 2003.
[44]
J. M. Salmon, “Interactions between yeast, oxygen and polyphenols during alcoholic fermentations: practical implications,” LWT—Food Science and Technology, vol. 39, no. 9, pp. 959–965, 2006.
[45]
S. Malherbe, F. F. Bauer, and M. du Toit, “Understanding problem fermentations–a review,” South African Journal of Enology and Viticulture, vol. 28, no. 2, pp. 169–186, 2007.
[46]
I. Mannazzu, D. Angelozzi, S. Belviso et al., “Behaviour of Saccharomyces cerevisiae wine strains during adaptation to unfavourable conditions of fermentation on synthetic medium: cell lipid composition, membrane integrity, viability and fermentative activity,” International Journal of Food Microbiology, vol. 121, no. 1, pp. 84–91, 2008.
[47]
E. Gómez-Plaza and M. Cano-López, “A review on micro-oxygenation of red wines: claims, benefits and the underlying chemistry,” Food Chemistry, vol. 125, no. 4, pp. 1131–1140, 2011.
[48]
M. Ciani, L. Ferraro, and F. Fatichenti, “Influence of glycerol production on the aerobic and anaerobic growth of the wine yeast Candida stellata,” Enzyme and Microbial Technology, vol. 27, no. 9, pp. 698–703, 2000.
[49]
S. Alfenore, C. Molina-Jouve, S. E. Guillouet, J. L. Uribelarrea, G. Goma, and L. Benbadis, “Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process,” Applied Microbiology and Biotechnology, vol. 60, no. 1-2, pp. 67–72, 2002.
[50]
A. I. El-Diwany, M. S. El-Abyad, A. H. El-Refai, L. A. Sallam, and R. F. Allam, “Effect of some fermentation parameters on ethanol production from beet molasses by Saccharomyces cerevisiae Y-7,” Bioresource Technology, vol. 42, no. 3, pp. 191–195, 1992.
[51]
Z. Kádár, Z. Szengyel, and K. Réczey, “Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol,” Industrial Crops and Products, vol. 20, no. 1, pp. 103–110, 2004.
[52]
E. Bellissimi and W. M. Ingledew, “Metabolic acclimatization: preparing active dry yeast for fuel ethanol production,” Process Biochemistry, vol. 40, no. 6, pp. 2205–2213, 2005.
[53]
K. G. Duodu, J. R. N. Taylor, P. S. Belton, and B. R. Hamaker, “Factors affecting sorghum protein digestibility,” Journal of Cereal Science, vol. 38, no. 2, pp. 117–131, 2003.
[54]
J. Pi？kur, E. Rozpedowska, S. Polakova, A. Merico, and C. Compagno, “How did Saccharomyces evolve to become a good brewer?” Trends in Genetics, vol. 22, no. 4, pp. 183–186, 2006.
[55]
T. O. Akinwale, “Fermentation and post fermentation chances in cashew wine,” The Journal of Food Technology in Africa, vol. 4, no. 3, pp. 100–102, 1999.
[56]
C. S. Yah and S. E. Iyuke, “Monitoring barley extract utilization by Saccharomyces cerevisiae in a Microbrewery,” Master Brewers Association of the Americas-MBAA TQ, 2010.
[57]
S. M. Araujo, C. F. Silva, J. J. S. Moreira, N. Narain, and R. R. Souza, “Biotechnological process for obtaining new fermented products from cashew apple fruit by Saccharomyces cerevisiae strains,” Journal of Industrial Microbiology Biotechnology, vol. 38, no. 9, pp. 1161–1169, 2011.
[58]
L. V. A. Reddy and O. V. S. Reddy, “Effect of fermentation conditions on yeast growth and volatile composition of wine produced from mango (Magnifera indica L.) fruit juice,” Food and Bioproducts Processing, vol. 89, no. 4, pp. 487–491, 2011.
[59]
W. C. Quayle, A. Fattore, R. Zandona, E. W. Christen, and M. Arienzo, “Evaluation of organic matter concentration in winery wastewater: a case study from Australia,” Water Science and Technology, vol. 60, no. 10, pp. 2521–2528, 2009.
[60]
J. C. Wang and H. H. Wang, “Fermentation products and carbon balance of spoilage Bacillus cereus,” Journal of Food and Drug Analysis, vol. 10, no. 1, pp. 64–68, 2002.
