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Effect of Grafting Dates, Methods on Success and Growth of Mandarin (Citrus reticulata Blanco) SaplingDOI: 10.3126/njst.v14i1.8873, PP. 23-30 Keywords: citrus,propagation,callus,cambium,mortality,graft success Abstract: A field experiment was conducted at National Citrus Research Program (NCRP), Paripatle, Dhankuta to standardize the time and method of grafting and to assess the success and growth of mandarin saplings. Scions taken from ‘Khoku Local’ mandarin were grafted onto one-year-old trifoliate orange seedling rootstocks by shoot tip and veneer methods in eight different dates starting from 29 th October, 2009 to 12 th February, 2010 at 15 days interval. The grafts were planted inside closed tunnel of jute and plastic sheet at 10×10 cm spacing in 50×60 cm 2 experimental plots in two factorial split-plot design with three replications per treatment, containing 30 grafts per plot. The grafting methods were allotted on main-plots and the dates on sub-plots randomly. The success and growth of sapling was significantly affected by the dates while the methods had no effect. Study results showed that the highest success (96.11 %) was found on 13 th January, followed by 28 th January (91.11%) and the least (51.67%) on 29 th October grafting. The mortality of sprouted grafts was the lowest (0.17%) in 29 th December grafting, while it was observed higher in earlier and late season grafting. Observation on growth taken at 180 days after grafting in successful grafts showed that 29 th October had the highest number of primary branches (2.2); 14 th November had the highest number of leaves (48.47) and secondary branches (2.505); 29 th December gave the maximum sapling height (32.86 cm); 13 th January produced the lowest number of leaves (21.93) per sapling and 28 th January produced the lowest number of primary (1.533) and secondary branches (1.172) per sapling. From the study it was revealed that in Dhankuta, the most appropriate time of grafting was 2 nd to 4 th week of January. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 23-30 DOI: http://dx.doi.org/10.3126/njst.v14i1.8873
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