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草业科学  2015 

蒺藜苜蓿EMS诱变突变体库的构建及突变体表型的分析

DOI: 10.11829\j.issn.1001-0629.2014-0034, PP. 71-77

Keywords: 蒺藜苜蓿,甲基磺酸乙酯,诱变

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Abstract:

?突变体是进行功能基因组学研究的重要材料,大量基因已经从各种突变体中得到克隆。本研究利用甲基磺酸乙酯(EMS)诱变蒺藜苜蓿(Medicagotruncatula)品种A17种子,鉴定和统计其后代突变型和突变频率,分别获得2085份M1代和388份M2代突变材料,M2代突变材料中叶色突变性状包括叶斑突变、叶深绿、杂色叶、叶红紫、叶黄萎和白化苗,占群体的5.37%;叶型突变性状包括皱叶、窄叶、卷曲和多叶等,占3.12%;株型突变性状包括高秆、矮化、匍匐、半匍匐、多分枝和少分枝等,占9.11%;开花期突变性状包括早花、晚花等,占1.01%;总的表型突变频率为18.61%。本研究初步构建的蒺藜苜蓿A17EMS的突变材料一方面创造了蒺藜苜蓿新的种质资源,另一方面可应用于蒺藜苜蓿功能基因组的研究。

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