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泥炭样品当中α-纤维素提取的实验方法探讨

, PP. 728-734

Keywords: 泥炭,&alpha,-纤维素,提取流程

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Abstract:

目前,泥炭α-纤维素同位素研究已广泛应用于古气候、古环境重建,因而泥炭α-纤维素的提取也就显得尤为重要,但国内外泥炭α-纤维素的提取方法存在差异,并未形成比较统一的α-纤维素提取流程,在一定程度上限制了各实验室之间泥炭α-纤维素同位素数据的对比分析。在前人树轮、湖泊和泥炭α-纤维素提取流程的基础之上进行改善,经过反复的条件实验,最终建立了适合泥炭岩芯样品的α-纤维素提取方法。对泥炭α-纤维素提取流程的改进主要表现在4个方面:(1)样品处理前增加了清洗步骤,并设计了简单的清洗装置,可以有效清除粘土和矿物等杂质。(2)α-纤维素提取自始至终是在10ml的Teflon离心管中进行,避免了在实验过程中多次转移样品而造成样品的损失,同时使用离心机分离缩短了样品的制备时间,并实现了α-纤维素的批量化提取。(3)NaClO2的漂白和不同浓度的NaOH溶液的碱洗取代有机溶剂的萃取。(4)α-纤维素提取流程中增加了样品的均一化处理,保证了测试样品具有较好的代表性。红外光谱分析结果证明提取的纤维素是比较纯的α-纤维素,对40个泥炭岩芯样品α-纤维素碳同位素的相对误差分析和线性回归分析,进一步强调在α-纤维素提取流程中样品均一化处理的必要性。

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