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核农学报  2015 

小麦旗叶形态相关性状的QTL定位

DOI: 10.11869/j.issn.100-8551.2015.07.1253, PP. 1253-1259

Keywords: 小麦,DH群体,旗叶,形态性状,QTL

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Abstract:

为了发掘控制小麦旗叶形态性状的QTL,本试验以旱选10号/鲁麦14构建的包含有150个株系的DH群体为材料,采用完全随机区组设计,通过基于混合线性模型复合区间作图法,在干旱胁迫和正常灌溉2种水分条件下对小麦开花期旗叶长、叶宽、叶面积进行QTL定位。结果表明,共检测到3个旗叶长、4个旗叶宽和5个旗叶面积QTL,贡献率为6.06%~14.19%。其中qFLW-7A-1和qFLA-7B-1在2种水分条件下均被检测到,这2个QTL的表达不依赖于水分条件;其余的10个QTL只在1种水分条件下检测到,表明其表达依赖于水分条件。在5D染色体的标记区间Xgdm43~Xgwm174上发现有同时存在控制旗叶长和叶面积的QTL,在7B染色体的标记区间Xgdm68.1~WMC269.1上检测到同时控制旗叶长、叶宽和叶面积的QTL。本研究旨在检测不同环境条件下能够稳定表达的小麦旗叶形态性状QTL,为进一步精细定位和图位克隆提供理论参考。

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