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核农学报  2015 

铅胁迫下萝卜基因组DNA甲基化分析

DOI: 10.11869/j.issn.100-8551.2015.07.1278, PP. 1278-1284

Keywords: 萝卜,铅胁迫,DNA甲基化,甲基化敏感扩增多态性

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Abstract:

利用甲基化敏感扩增多态性(MSAP)技术,分析不同浓度铅(Pb)处理下萝卜基因组DNA甲基化水平的变化,探讨DNA甲基化在植株对Pb胁迫反应中的作用。结果表明,经100、400和800mg·L-1Pb(NO3)2处理后,MSAP比率分别为12.7%、14.2%和17.0%,均高于对照;全甲基化率分别为9.7%、10.2%和12.8%,而其对照为9.5%,表明重金属铅胁迫后,某些位点发生了重新甲基化。萝卜肉质根中总甲基化水平随Pb处理浓度上升而提高。甲基化变异可分为重新甲基化、去甲基化、不定类型以及与对照相同等甲基化类型。萝卜Pb胁迫处理引起的植株基因组甲基化程度的提高主要是重新甲基化。研究为从DNA水平揭示重金属Pb毒害机理及植物对Pb胁迫的响应机制提供了理论依据。

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