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核农学报  2015 

马铃薯基因组中Dof转录因子家族的鉴定与表达特征分析

DOI: 10.11869/j.issn.100-8551.2015.07.1260, PP. 1260-1270

Keywords: 马铃薯,Dof转录因子,生物信息学,基因表达

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Abstract:

Dof(DNA-bindingwithonezincfinger)锌指蛋白是植物特有的一类转录因子,在多种代谢途径中发挥着重要作用。为全面了解Dof基因在马铃薯基因组中的特征,利用生物信息学方法鉴定出了马铃薯Dof基因家族成员,并对基因的结构、染色体定位、系统进化关系和保守结构域进行了预测,分析了Dof基因在不同组织和系列胁迫下的表达特征。结果表明,马铃薯Dof转录因子家族由35个基因编码43个蛋白质;基因分布于除7号和12号染色体外的其余10条染色体上;STDOF蛋白长度在165~503个氨基酸之间,等电点介于4.72~10.04;系统发生分析表明,STDOF蛋白分为4个亚族;转录组数据分析显示,StDof基因的表达具有组织特异性,在不同胁迫条件下,多数StDof基因的表达也呈现有不同程度的差异。本研究为深入探讨StDof基因的功能及在马铃薯的遗传改良中的应用奠定了基础。

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