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核农学报  2015 

利用45Ca探究BnTR1基因对油菜Ca2+吸收的影响

DOI: 10.11869/j.issn.100-8551.2015.07.1366, PP. 1366-1373

Keywords: BnTR1,45Ca,同位素示踪,原生质体,油菜

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Abstract:

为探索钙离子与油菜胁迫响应机制的关系,利用45Ca放射性示踪剂研究基因BnTR1(BrassicanapusThermalResistantgene1)对油菜钙吸收的影响。试验分析了从BnTR1转基因油菜和野生型油菜中提取出来的原生质体对钙离子的吸收,比较其差异。结果表明,当胞外钙离子浓度低于0.9mmol·L-1时,转基因型原生质体比野生型原生质体吸收更多的钙离子;当外界钙离子浓度高于1mmol·L-1时,转基因型原生质体却比野生型原生质体吸收的钙离子更少;当胞外钙离子浓度大于30mmol·L-1时,转基因型原生质体吸收的钙离子大大低于野生型原生质体的吸收量;当加入钙离子通道阻塞剂氯化镧且其浓度从5μmol·L-1上升到20μmol·L-1时,转基因型原生质体会出现钙离子吸收峰值。综上,BnTR1基因通过对细胞膜上钙离子通道更为灵敏和有效的调控,可以提高油菜对钙离子胁迫的耐受性。研究结果对研究BnTR1基因的作用机制,以及其对油菜耐胁迫能力的影响提供了新的方向。

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