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棉花学报  2015 

棉花S-腺苷蛋氨酸脱羧酶基因(GhSAMDC2/3/4)的克隆及其诱导表达分析

DOI: 10.11963/issn.1002-7807.201502011, PP. 176-183

Keywords: 棉花,多胺,S-腺苷蛋氨酸脱羧酶基因,非生物胁迫

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Abstract:

利用电子克隆结合RT-PCR技术克隆获得陆地棉(GossypiumhirsutumL.)S ̄腺苷蛋氨酸脱羧酶(S-adenosylmethioninedecarboxylase,SAMDC)基因家族3个基因,分别命名为GhSAMDC2、GhSAMDC3和GhSAMDC4。序列分析显示,该基因cDNA包含的upstreamORF(uORF)和mainORF(mORF)为植物SAMDC基因特征ORF,其中mORF长度分别为1068bp、1110bp和1032bp,分别编码355、369和343个氨基酸。聚类分析表明,GhSAMDC2/3蛋白与可可树(Theobromacacao)SAMDC聚为一类,且GhSAMDC2与GhSAMDC3蛋白亲缘关系最近;GhSAMDC4与拟南芥AtSAMDC4聚为一类。实时荧光定量PCR分析表明,GhSAMDC2在茎中表达相对较高,随着纤维发育其表达量不断增加,在纤维发育后期其表达量达到最高;GhSAMDC2/3/4在不同的胁迫条件下表现出不同的表达模式,GhSAMDC2受低温和干旱胁迫诱导最强烈,GhSAMDC3响应盐胁迫显著,GhSAMDC4受ABA诱导强烈。上述结果为进一步研究棉花SAMDC基因功能奠定了一定基础。

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