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棉花学报  2013 

棉花纤维发育早期RNA-Seq转录组分析

DOI: 1002-7807(2013)03-0189-08, PP. 189-196

Keywords: 棉纤维发育早期,RNA-Seq,qRT-PCR

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Abstract:

为了揭示棉花纤维发育早期基因表达变化情况,本研究以纤维长度存在显著差异的两个陆海回交近交系NMGA-062(32.58mm)和NMGA-105(27.06mm)为材料,利用IlluminaHiSeqTM2000对0、3DPA(Dayspostanthesis)的胚珠及10DPA的纤维进行RNA-Seq测序。六个文库进行拼接,共得到长度大于200bp的Unigene98464个,总长度约为88.2Mb。对10DPA的纤维转录组数据进行差异表达分析,共筛选到1931个差异表达基因,1536个Unigene上调,395个Unigene下调。GO(Geneontology)功能显著性富集和Pathway显著性富集分析发现,差异表达基因富集在脂质转移活性(Lipidtransportactivity)分子功能组和脂质代谢通路(Lipidmetabolismpathway),由此推测脂类相关基因可能在纤维伸长发育过程中起重要作用。通过对棉纤维发育10DPA基因转录水平差异比较分析,为深入开展纤维伸长相关功能基因的克隆和功能验证提供了丰富的资源,并为揭示棉花纤维伸长的机制打下了坚实的基础。

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