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棉花学报  2015 

VIGS技术及其在棉花功能基因组研究中的应用进展

DOI: 10.11963/issn.1002-7807.201505011, PP. 469-473

Keywords: 棉花,VIGS,基因功能,抗病基因,品质基因

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Abstract:

病毒诱导的基因沉默(Virusinducedgenesilencing,VIGS)是一种转录后基因沉默现象,是植物体内普遍存在的遗传免疫机制,现已被开发为快速、高效、高通量的反向遗传学技术,在植物基因功能研究中得到广泛应用。近年,利用VIGS技术进行棉花基因功能研究也取得了一定进展。本文对VIGS技术的发展、操作技术的优化进行了综述,尤其总结了VIGS技术在棉花抗病、品质改良、生长发育等基因鉴定和功能研究中的应用进展,并对其应用前景进行了展望。

References

[1]  null Wang Kunbo, Wang Zhiwen, Li Fuguang, et al. The draft genome of a diploid cotton Gossypium raimondii[J]. Nature Genetics, 2012, 44(10): 1098-1103.
[2]  Paterson A H, Wendel J F, Gundlach H, et al. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres[J]. Nature, 2012, 492(7429): 423-427.
[3]  Liu Yule, Schiff M, Czymmek K, et al. Autophagy regulates programmed cell death during the plant innate immune response[J]. Cell, 2005, 121: 567-577.
[4]  Tameling W I, Baulcombe D C. Physical association of the NB-LRR resistance protein Rx with a Ran GTPase-activating protein is required for extreme resistance to potato virus X[J]. Plant Cell, 2007, 19: 1682-1694.
[5]  Kang Y W, Kim R N, Cho H S, et al. Silencing of a BYPASS1 homolog results in root-independent pleiotrophic developmental defects in Nicotiana benthamiana[J]. Plant Molecular Biology, 2008, 68: 423-437.
[6]  Bouvier F, Linka N, Isner J C, et al. Arabidopsis SAMT1 defines a plastid transporter regulating plastid biogenesis and plant development[J]. Plant Cell, 2006, 18: 3088-3105.
[7]  van Kammen A. Virus-induced gene silencing in infected and transgenic plants[J]. Trends in Plant Science, 1997, 2: 409-411.
[8]  Purkayastha A, Dasgupta I. Virus-induced gene silencing: a versatile tool for discovery of gene functions in plants[J]. Plant Physiology Biochemistry, 2009, 47: 967-976.
[9]  Kumagai M H, Donson J, Della-Cioppa G, et al. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA[J]. Proceeding of the National Academy of Sciences, 1995, 92: 1679-1683.
[10]  Ruiz M T, Voinnet O, Baulcombe D C. Initiation and maintenance of virus induced gene silencing[J]. Plant Cell, 1998, 10: 937-946.
[11]  Ratcliff F, Martin-Hernandez A M, Baulcombe D C. Tobacco rattle virus as a vector for analysis of gene function by silencing[J]. Plant Journal, 2001, 25: 237-245.
[12]  Liu Yule, Schiff M, Marathe R, Dinesh-Kumar S P. Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus[J]. Plant Journal, 2002, 30: 415-429.
[13]  Peele C, Jordan C V, Muangsan N, et al. Silencing of a meristematic gene using geminivirus-derived vectors[J]. Plant Journal, 2001, 27: 357-366.
[14]  Zhang Chunquan, Ghabrial S A. Development of bean pod mottle virus-based vectors for stable protein expression and sequence-specific virus-induced gene silencing in soybean[J]. Virology, 2006, 344(2): 401-411.
[15]  Hayward A, Padmanabhan M, Dinesh-Kumar S P. Virus-induced gene silencing in Nicotiana benthamiana and other plant species[J]. Plant Reverse Genetics: Methods and Protocols, Methods in Molecular Biology, 2011, 678: 55-63.
[16]  Burch-smith T M, Schiff M, Liu Yule, et al. Efficient virus-induced gene silencing in Arabidopsis[J]. Plant Physiology, 2006, 142: 21-27.
[17]  Liu Yule, Schiff M, Dinesh-Kumar S P. Virus-induced gene silencing in tomato[J]. Plant Journal, 2002, 31: 777-786.
[18]  Chung E, Seong E, Kim Y C, et al. A method of high frequency virus-induced gene silencing in chili pepper (Capsicum annuum L. cv. Bukang)[J]. Molecular Cells, 2004, 17: 377-380.
[19]  Valentine T, Shaw J, Blok V C, et al. Efficient virus-induced gene silencing in roots using a modified tobacco rattle virus vector[J]. Plant Physiology, 2004, 136: 3999-4009.
[20]  Dong Yiyu, Burch-Smith T M, Liu Yule, et al. A ligation-independent cloning TRV vector for high-throughput virus induced gene silencing identifies roles for NbMADS4-1 and -2 in floral development[J]. Plant Physiology, 2007, 145(4): 1161-1170.
[21]  Turnage M A, Muangsna N, Peele C Q, et al. Geminiviurs-based vectors of gene silencing in Arabidopsis[J]. Plant Journal, 2002, 30(l): 107-114.
[22]  Kharazmi S, Behjatnia S A A, Hamzehzarghani H, et al. Cotton leaf curl Multan betasatallite as a plant gene delivery vector trans- activated by taxonomically diverse geminiviruses[J]. Archives of Virology, 2012, 157:1269-1279.
[23]  Jeyabharathy C, Shakila H, Usha R. Development of a VIGS vector based on the β-satellite DNA associated with bhendi yellow vein mosaic virus[J]. Virus Research, 2015, 195: 73-78.
[24]  Burch-smith T M, Anderson J C, Martin G B, et al. Applications and advantages of virus-induced gene silencing for gene function studies in plants[J]. Plant Journal, 2004, 39: 734-746.
[25]  王心宇, 吕坤, 蔡彩平, 等. TRV病毒介导的基因沉默体系在棉花中的建立及应用[J]. 作物学报, 2014, 40(8): 1356-1363.
[26]  Wang Xinyu, Lü Kun, Cai Caiping, et al. Establishment and application of TRV-mediated virus-induced gene silencing in cotton[J]. Acta Agronomica Sinica, 2014, 40(8): 1356-1363.
[27]  Gao Xiquan, Robert C, Britt J, et al. Agrobacterium-mediated virus-induced gene silencing assay in cotton[J]. Journal of Visualized Experiments, 2011, 54: 2983.
[28]  Bartel D P. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116: 281-297.
[29]  Thomas C L, Jones L, Baulcombe D C, et al. Size constraints for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana bethamiana using a potato virus X vector[J]. Plant Journal, 2001, 25: 417-425.
[30]  Gao Xiquan, Wheeler T, Li Zhaohu, et al. Silencing GhNDR1 and GhMKK2 compromises cotton resistance to Verticillium wilt[J]. Plant Journal, 2011, 66: 293-305.
[31]  Gao Xiquan, Li Fangjun, Li Maoying, et al. Cotton GhBAK1 mediates Verticillium wilt resistance and cell death[J]. Journal of Integrative Plant Biology, 2013, 55(7): 586-596.
[32]  Qu Jing, Jian Ye, Geng Y F, et al. Dissecting functions of KATANIN and WRINKLED1 in cotton fiber development by virus-induced gene silencing[J]. Plant Physiology, 2012, 160(2): 738-48.
[33]  Forfana I B F, Sangare A, Collier R, et al. A geminivius-induced gene silencing system for gene function validation in cassava[J]. Plant Molecular Biology, 2004, 56: 613-624.
[34]  Tuttle J R, Haigler C H, Robertson D. Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system[J]. Plant Methods, 2012, 8: 27.
[35]  Cai Caiping, Zhang Xueying, Niu E, et al. GhPSY, a phytoene synthase gene, is related to the red plant phenotype in upland cotton (Gossypium hirsutum L.)[J]. Molecular Biology Reports, 2014, 41(8): 4941-4952.
[36]  王丽, 穆春, 张明才, 等. GhCPS基因沉默对棉花幼苗生长和内源激素含量的影响[J]. 棉花学报, 2014, 26(3): 189-196.
[37]  Wang Li, Mu Chun, Zhang Mingcai, et al. Effect of silencing GhCPS on the growth and endogenous hormone content of cotton seedlings(Gossypium hirsutum)[J]. Cotton Science, 2014, 26(3): 189-196.
[38]  Ekengren S K, Liu Y, Schiff M, et al. Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto-mediated disease resistance in tomato[J]. Plant Journal, 2003, 36: 905- 917.
[39]  Ryu C M, Anand A, Kang L, et al. Agrodrench: a novel and effective agroinoculation method of virus-induced gene silencing in roots and diverse solanaceous species[J]. Plant Journal, 2004, 40: 322-331.
[40]  Senthil-kumar M, Mysore K S. Virus-induced gene silencing can persist for more than 2 years and also be transmitted to progeny seedlings in Nicotiana benthamiana and tomato[J]. Plant Biotechnology Journal, 2011, 9: 797-806.

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