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植物学报  2015 

组培增殖方式对网纹草嵌合性状稳定性的影响

DOI: 10.3724/SP.J.1259.2015.00372, PP. 372-377

Keywords: 嵌合体,组培增殖,茎尖分生组织,稳定性,细胞谱系

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Abstract:

?以一种具有良好的谱系细胞标记的周缘嵌合体网纹草(Fittoniaalbivenis)为研究材料,对不同增殖方式下其组培苗嵌合性状的稳定性进行了观察分析。结果表明,以茎段诱导腋芽增殖时网纹草的稳定性很高,但以丛生芽增殖时其嵌合性状发生了变异,变异率为21.32%;叶片组织解剖学观察结果显示,母本嵌合体茎尖分生组织中红和绿2种细胞谱系的细胞层排布在丛生芽再生过程中发生了改变,这可能是导致新类型嵌合体产生的原因。研究结果不仅对植物嵌合体种苗的组培生产具有重要的指导意义,而且为嵌合体的种质创新工作提供了新方法。

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