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植物学报  2015 

花生重要农艺及产量性状的全基因组关联分析

DOI: 10.11983/CBB14144, PP. 460-472

Keywords: 花生,产量性状,SSR,全基因组关联分析

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Abstract:

?通过关联分析法发掘与花生(Arachishypogaea)产量性状显著关联、同时又在花生基因组上随机分布的SSR位点及优异等位变异,可了解产量相关基因区域的分布特点,有助于利用分子标记辅助选择方法选育高产花生新品种。选用64个SSR标记,采用MLM(Q+K)方法对166份花生资源进行全基因组关联分析。结果表明,通过聚类分析和结构划分,供试群体受其综合性状遗传特点和来源地域的影响可被划分成7个亚群,聚类结果与群体结构基本一致,同时群体特点与材料来源地的生态划分符合同类聚集的规律。通过对6个产量相关性状的3年数值的关联分析,分别发掘出SSR位点有20个、33个和26个,2年以上重复检出的SSR位点有13个(P<0.05),各SSR位点的表型变异解释率范围为0.011-0.3481,平均为0.0673;共检出590个等位变异,平均每个标记位点12.29个,表型变异解释率值最高的是与单株果数呈显著关联的位点TC1A02(P<0.001),含21个等位变异;与产量构成主要因子紧密关联的位点中,百果重的TC1A02-C470(+41.5885)、TC1A02-C560(+40.9261)和pPGPseq2E6-B473(+63.9534),单株果数的TC1A02-C500(+7.3744),单株饱果数的GM1843-E157(+4.3166),可用于产量性状的分子辅助育种。

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