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植物学报  2015 

外源Ca2+对PEG处理下转C4型PEPC基因水稻光合生理的调节

DOI: 10.3724/SP.J.1259.2015.00206, PP. 206-216

Keywords: 钙离子,高表达转PEPC基因水稻,干旱胁迫,磷酸烯醇式丙酮酸羧化酶,光合特性,水稻

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Abstract:

?磷酸烯醇式丙酮酸羧化酶(PEPC)通过固定二氧化碳参与光合作用,是关键的C4植物光合作用酶。为了揭示高光效转C4PEPC基因水稻(Oryzasativa)对干旱胁迫的适应机理,以高表达转C4PEPC水稻(PC)和野生型水稻Kitaake(WT)为供试材料,在植株的4-5叶期,使用不同浓度外源CaCl2溶液处理,测定在15%聚乙二醇6000(polyethyleneglycol-6000,PEG-6000)胁迫下叶片相对含水量、光合参数、内源钙总含量、叶片总蛋白激酶活性、PEPC酶活性以及相关基因表达和蛋白质含量。结果表明,0.5mmol?L-1CaCl2明显提高PC叶片相对含水量(P<0.05),2mmol?L-1和10mmol?L-1CaCl2则作用不显著,对WT则影响不显著。不同浓度钙处理对PEG处理PC的净光合速率影响不显著,而通过维持气孔导度减少水分胁迫。内源总钙浓度的数据显示,在PEG6000处理下,PC具有维持稳定内源Ca2+浓度的能力,过高浓度(10mmol?L-1CaCl2)钙处理反而降低了PEPC酶活性、PEPC基因表达和可溶性蛋白的含量。

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