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植物学报  2015 

PI3P在暗诱导的蚕豆气孔关闭中的作用及与H2O2和NO的关系

DOI: 10.11983/CBB15062, PP. 583-590

Keywords: 黑暗,过氧化氢,一氧化氮,磷脂酰肌醇3-磷酸,气孔关闭,蚕豆

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Abstract:

?以蚕豆(Viciafaba)表皮条为材料,利用磷脂酰肌醇3-激酶(PI3K)的抑制剂沃曼青霉素(WM)和LY294002(LY)抑制磷脂酰肌醇3-磷酸(PI3P)的形成,并结合气孔开度分析及激光扫描共聚焦显微镜技术,探讨暗诱导蚕豆气孔关闭过程中PI3P与过氧化氢(H2O2)和一氧化氮(NO)之间的相互关系。结果表明,WM和LY显著抑制暗诱导的保卫细胞H2O2和NO的形成以及气孔的关闭,但不能抑制外源H2O2和NO诱导的气孔关闭,外源H2O2和NO处理能完全逆转WM和LY对暗诱导的气孔关闭的抑制效应。实验结果暗示,在暗诱导的气孔关闭的信号转导途径中PI3P在信号分子H2O2和NO的上游起作用。

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