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葡萄COR27基因的克隆与抗寒功能研究

DOI: 10.11913/PSJ.2095-0837.2015.30346, PP. 346-354

Keywords: 葡萄,COR27基因,冷处理,抗寒性,转基因拟南芥

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Abstract:

?冬季低温是制约我国葡萄和葡萄酒产业发展的主要因素之一,揭示葡萄在冷胁迫下的信号转导通路、挖掘抗寒相关基因并解析其功能,对高耐寒品种的培育具有重要的理论和应用价值。本研究在欧亚种‘玫瑰香’葡萄(VitisviniferaL.‘MuscatHamburg’)冷胁迫相关转录组分析的基础上,鉴定了一个抗寒候选基因,通过同源性分析将其命名为VvCOR27。VvCOR27基因的cDNA序列(1082bp)中,其开放阅读框(ORF)为909bp,编码302个氨基酸。同源性分析显示,13个物种的COR27蛋白均具有3个特有的保守结构域。定量RT-PCR分析表明,VvCOR27在4℃低温处理24h后大量表达。基于基因组序列的启动子基序分析表明,VvCOR27启动子区均只含有1个EE、EEL、G-box、ABREL元件,其数量少于AtCOR27,这可能是VvCOR27响应冷胁迫较AtCOR27滞后的原因。对3个超表达VvCOR27转基因拟南芥株系的抗寒性鉴定表明,VvCOR27参与了植株对冷胁迫的应答并作为正调控因子增强了植株对冷胁迫的耐受能力。

References

[1]  贺普超. 葡萄学[M]. 北京:中国农业出版社, 2001.
[2]  孔庆山. 中国葡萄志[M]. 北京:中国农业科技出版社, 2004.
[3]  宋润刚, 路文鹏, 王军, 沈育杰, 林兴桂, 葛玉香, 李晓红, 孙克娟. 山葡萄品种选育回顾与展望[J]. 北方园艺, 1999, 129: 36-38.
[4]  林兴桂. 我国酿酒葡萄抗寒育种的回顾与展望[J]. 果树学报, 2007, 24(1): 89-93.
[5]  刘军, 王小伟, 魏钦平, 鲁韧强, 高照全. 世界葡萄抗寒育种的成就与展望[J]. 果树学报, 2004, 21(5): 461-466.
[6]  贺普超, 牛立新. 我国葡萄属野生种抗寒性的研究[J]. 园艺学报, 1989, 11(1): 81-88.
[7]  Jin WM, Dong J, Hu YL, Lin ZP, Xu XF, Han ZH. Improved cold-resistant performance in transgenic grape (Vitis vinifera L.) overexpressing cold-inducible transcription factors AtDREB1b[J]. Hortscience, 2009, 44(1): 35-39.
[8]  Tillett LR, Wheatley MD, Tattersall EAR, Schlauch KA, Cramer GR, Cushman JC. The Vitis vinifera C-repeat binding protein 4 (VvCBF4) transcriptional factor enhances freezing tolerance in wine grape[J]. Plant Biotechnology J, 2012, 10(1): 105-124.
[9]  Zhang JZ, Creelman RA, Zhu JK. From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops[J]. Plant Physiol, 2004, 135(2): 615-621.
[10]  Zhou MQ, Shen C, Wu LH, Tang KX, Lin J. CBF-dependent signaling pathway: a key responder to low temperature stress in plants[J]. Crit Rev Biotechnol, 2011, 31(2): 186-92.
[11]  Xiao H, Siddiqua M, Braybrook S, Nassuth A. Three grape CBF/DREB1 genes respond to low temperature, drought and abscisic acid[J]. Plant Cell Environ, 2006, 29(7): 1410-1421.
[12]  Xiao H, Tattersall EA, Siddiqua MK, Cramer GR, Nassuth A. CBF4 is a unique member of the CBF transcription factor family of Vitis vinifera and Vitis riparia[J]. Plant Cell Environ, 2008, 31(1): 1-10.
[13]  Li JT, Wang LN, Zhu W, Wang N, Xin HP, Li SH. Characterization of two VvICE1 genes isolated from ‘Muscat Hamburg’ grapevine and their effect on the tolerance to abiotic stresses[J]. Sci Hortic, 2014a, 165(22): 266-273.
[14]  Li JT, Wang N, Wang LN, Xin HP, Li SH. Molecular cloning and characterization of the HOS1 gene from ‘Muscat Hamburg’ grapevine[J]. J Am Soc Hortic Sci, 2014b, 139(1): 54-62.
[15]  Liu LY, Li H. Research progress in amur grape, Vitis amurensis Rupr.[J]. Can J Plant Sci, 2013, 93(4): 565-575.
[16]  Dong C, Tao JM, Zhang M, Qin Y, Yu ZY, Wang BL, Cai BH, Zhang Z. Isolation and expression characterization of CBF2 in Vitis amurensis with stress[J]. Agr Sci, 2013a, 4(9): 466-472.
[17]  Dong C, Zhang M, Yu ZY, Ren JP, Qin Y, Wang BL, Xiao LZ, Zhang Z, Tao JM. Isolation and expression analysis of CBF4 from Vitis amurensis associated with stress[J]. Agr Sci, 2013b, 4(5): 224-229.
[18]  Dong C, Zhang Z, Qin Y, Ren JP, Huang JF, Wang BL, Lu HL, Cai BH, Tao JM. VaCBF1 from Vitis amurensis associated with cold acclimation and cold tolerance[J]. Acta Physiol Plant, 2013c, 35(10): 2975-2984.
[19]  Dong C, Zhang Z, Ren JP, Qin Y, Huang JF, Wang Y, Cai BH, Wang BL, Tao JM. Stress-responsive gene ICE1 from Vitis amurensis increases cold tolerance in tobacco[J]. Plant Physiol Bioch, 2013d, 71(2013): 212-217.
[20]  Li JT, Wang N, Xin HP, Li SH. Overexpression of VaCBF4, a transcription factor from Vitis amurensis, improves cold tolerance accompanying increased resistance to drought and salinity in Arabidopsis[J]. Plant Mol Biol Rep, 2013, 31(6): 1518-1528.
[21]  Mikkelsen MD, Thomashow MF. A role for circa-dian evening elements in cold-regulated gene expression in Arabidopsis[J]. Plant J, 2009, 60(2): 328-339.
[22]  Kilian J, Whitehead D, Horak J, Wanke D, Weinl S, Batistic O, D''Angelo C, Bornberg-Bauer E, Kudla J, Harter K. The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses[J]. Plant J, 2007, 50(2): 347-363.
[23]  Xin HP, Zhu W, Wang LN, Xiang Y, Fang LC, Li JT, Sun XM, Wang N, Londo J, Li SH. Genome wide transcriptional profile analysis of Vitis amurensis and V. vinifera in response to cold stress[J]. PLoS One, 2013, 8(3): e58740.
[24]  Xu W, Li R, Zhang N, Ma F, Jiao Y, Wang Z. Transcriptome profiling of Vitis amurensis, an extremely cold-tolerant Chinese wild Vitis species, reveals candidate genes and events that potentially connected to cold stress[J]. Plant Mol Biol, 2014, 86(4-5): 527-541.

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