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盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析

DOI: 10.11913/PSJ.2095-0837.2015.40499, PP. 499-506

Keywords: 盐穗木,钾转运蛋白基因HcKUP12,克隆,盐胁迫,表达分析

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Abstract:

?利用RACE技术从盐生植物盐穗木(Halostachyscaspica)中克隆获得了一个钾离子转运体基因,经BLAST同源比对发现该基因与拟南芥钾离子转运蛋白AtKUP12基因最为相似,命名为HcKUP12。HcKUP12基因cDNA全长为2953bp,含有2544bp的阅读框、262bp的5'-UTR和147bp的3'-UTR,编码847个氨基酸,分子质量为93.31kD,理论等电点为7.19。利用实时荧光定量RT-PCR分析了HcKUP12基因在600mmol/LNaCl处理下胁迫24h的表达模式,结果显示该基因在盐胁迫下表达量显著增加,至处理24h时达到最高(为对照的225倍),初步推测其可能是与盐穗木耐盐相关的一个候选基因。

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