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分析化学  2015 

化学发光酶免疫分析测定鱼肉中呋喃它酮代谢物方法研究

DOI: 10.11895/j.issn.0253-3820.150129, PP. 871-875

Keywords: 呋喃它酮,5-吗啉甲基-3-氨基-2-GFDA1唑烷基酮,间接竞争化学发光酶免疫分析,多克隆抗体,鱼肉

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Abstract:

建立了呋喃它酮代谢物5-吗啉甲基-3-氨基-2-GFDA1唑烷基酮(AMOZ)间接竞争化学发光酶免疫分析(icCLEIA)检测方法,通过单因素实验优化了包被原浓度、抗体稀释倍数、反应缓冲体系及浓度、竞争反应时间等参数,结果表明icCLEIA最佳反应条件为:包被抗原浓度为10ng/mL,抗体稀释60000倍,最佳竞争时间为50min,体系缓冲液0.01mol/LPBS(pH7.4).在优化的条件下,本方法的线性检测范围为0.026~3.52μg/L,IC50为0.29μg/L,检出限(LOD,IC10)为0.012μg/L.对鱼肉样品的平均添加回收率在101.4%~115.5%之间.建立的icCLEIA方法可用于实际样品中AMOZ残留检测.

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