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灰飞虱保幼激素结合蛋白基因的克隆及表达动态

DOI: 10.7685/j.issn.1000-2030.2012.02.010, PP. 59-64

Keywords: 灰飞虱,保幼激素结合蛋白,基因克隆,表达动态,qRT-PCR

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Abstract:

克隆了灰飞虱的1个保幼激素结合蛋白(JHBP)基因,命名为LstrJHBP(GenBank登录号JF728808)。该基因全长1514bp,开放阅读框编码289个氨基酸,5’和3’UTR区分别为124和522bp,预测的相对分子质量为32599,等电点5.47。综合信号肽预测、氨基酸序列比对及聚类分析结果,推测LstrJHBP是一个细胞质JHBP。qRT-PCR测定表明,LstrJHBP在灰飞虱若虫5个龄期及雌雄成虫均有表达,但4龄若虫期显著高于其他龄期,雌雄成虫间没有显著差异。研究结果为进一步明确灰飞虱的发育及变态调控机制奠定了基础。

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