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猪嵴病毒CH441株VP1基因的克隆与序列分析

DOI: 10.7668/hbnxb.2015.02.014, PP. 72-77

Keywords: VP1基因,克隆,DNA测序,序列分析

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Abstract:

为了深入研究嵴病毒(swKoV)主要结构蛋白基因VP1,根据GenBank中已发表的猪嵴病基因序列设计特异性引物,采用RT-PCR方法扩增猪嵴病毒CH441株VP1基因,并对其进行克隆与测序分析。结果表明,swKoVCH441株的VP1基因为762bp,与GenBank已发表的嵴病毒属的15株嵴病毒序列的VP1基因相比较,swKoVCH441株的VP1基因与其他各毒株VP1基因的核苷酸同源性为81.5%~90.2%,氨基酸同源性为86.6%~96.9%,进化分析显示,swKoVCH441株与GS-1株之间的亲缘关系较近。生物信息学分析显示,VP1蛋白理论等电点(pI)为4.40,理论分子质量为26.9782kDa;其序列上共发现18个磷酸化位点,分别为Ser(7)、Thr(6)和Tyr(5),而蛋白的磷酸化与信号转导有关,预测该蛋白为一重要的信号转导分子;无信号肽和跨膜区。为进一步开展swKoVCH441株VP1基因在遗传变异等方面的研究奠定了理论基础。

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