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鸡Vezf1基因RCAS干扰载体构建及鉴定

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.08.015, PP. 102-107

Keywords: RCAS载体,RNAi,Vezf1,鸡胚

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Abstract:

为研究Vezf1基因在鸡胚早期发育过程中的作用,构建了针对鸡Vezf1基因的RCAS病毒RNA干扰载体,分别在鸡胚细胞和胚胎水平对Vezf1基因实施沉默,通过Real-timePCR和原位杂交法检测目的基因mRNA表达水平。结果表明,基于RCAS病毒的干扰载体可以在鸡胚成纤维细胞和活体鸡胚中成功沉默Vezf1基因的表达。此研究为利用鸡胚模型深入了解Vezf1基因在早期发育过程中的作用提供了素材。

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