To investigate the effect of mitochondrial DNA
deletion and OSW-1 on PI3K-AKT signaling pathway PCR Array in SK-Hep1
hepatocellular carcinoma cells, we prepared
SK-Hep1 cells with mtDNAdeletion, that is, p0SK Hep. Then the OSW-1 of 100ng/L was used to intervene SK-Hep1 and p0SK-Hep1.
RT-qPCR was used to detect the difference of gene expression onPI3K-AKT signaling pathway
PCR Arrayin four
groups of cells. The gene expression of TLR4, FOS and TSC2 markersin SK-Hep1 cells treated
with OSW-1 were significantly increased. The gene expressions of PDPK1, GJA1, TLR4 and TSC2 markerswere significantly increased in p0SK-Hep1 cells, and the gene
expressions of IRAK1
and GJA1 markers were significantly increased in p0SK-Hep1 cells
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