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-  2019 

C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction

DOI: 10.1038/s41419-019-1760-5

Keywords: Cell delivery, Mesenchymal stem cells, Myocardial infarction

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Abstract:

a, b Surface marker molecules expressed in passage 4 MSCs were analyzed by flow cytometry and quantified by image analysis of positive cells. Data are means?±?SEMs (n?=?4). c Representative image of MSCs morphology (passage 4) and multilineage differentiation capacity into osteoblasts and adipocytes, Images were at original magnification, ×100. d RT-PCR was used to evaluate CTRP3 mRNA levels in MSCs exposed to serum deprivation and hypoxia (n?=?4). e, f Western blotting and quantification of CTRP3 in MSCs (n?=?6). g RT-PCR analysis for MSCs treated with serum deprivation plus 400?μM H2O2 for 24?h (n?=?4). h Western blotting and quantification of CTRP3 in MSCs cell lysates (n?=?6). i Western blots and quantification of CTRP3 in conditioned medium (CM) from MSCs treated with serum deprivation plus H2O2 (n?=?5). Data are means?±?SEMs. *P?<?0.05; **P?<?0.01; ***P?<?0.001 versus the contro

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