Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance

a The mice-breeding scheme for gametogenesis study was shown. b Bright-field and Oct4-GFP fluorescence image of primary BMDCs derived from p53？/？ and p53+/+ mice were shown. c Oct4-GFP fluorescence, Nanog staining image, and DAPI staining image showed the morphology and germ cell marker expression of germ cell-like cells in p53？/？ BMDCs. d Oct4-GFP fluorescence and meiosis marker SCP3 staining image in p53？/？ BMDCs. e Bright-field, Oct4-GFP fluorescence image and DAPI staining of p53？/？ BMDCs showed the oocyte-like cells with germinal vesicle (GV)-like structures (black arrow) and oocyte-like cells with a polar body (PB)-like structure (white arrow). f The qRT-PCR for a series of germ cell markers, including early germ cell markers (Oct4, Sox2, Nanog, Stellar, Nanos3, and Ifitm3), meiosis markers (Scp1, Scp3, and Dmc1), and late germ cell markers (Vasa, Dazl, Oog1, and Nobox) in paired primary BMDCs derived from p53？/？ and p53+/+ mice (n？=？3). **p？<？0.01. g Bright-field and Oct4-GFP fluorescence image showed the embryo-like structures at the different developmental stages in p53？/？ BMDCs were seen after sub-culturing at different culture times. h Oct4-GFP+ (100 cells) and Oct4-GFP？ (100 cells) cells isolated from p53？/？ BMDCs or p53+/+ BMDCs (~500？000 cells) with six passage culture were injected subcutaneously into nude mice (5 mice/each group) for tumorigenicity assay (n？=？5). **p？<？0.01. The representative tumor sections from Oct4-GFP+ p53？/？ BMDCs stained with hematoxylin and eosin (H&E). The oocyte-like tumor cell was indicated with arrow (scale bar？=？20？μm