Integrative single-cell analysis of transcriptome, DNA methylome and chromatin accessibility in mouse oocytes

Single-cell analysis of both chromatin accessibility and DNA methylation in growing mouse oocytes. a A diagram of the number and diameters of growing oocytes used at each stage. The lower panel shows the mean of chromatin accessibility (GCH methylation level), DNA methylation (WCG methylation level) and the abundance of transcripts at each stage. b Comparison of the mapping efficiency among iscCOOL-seq, sci-MET34, snmC-seq33, scCOOL-seq via the PBAT strategy and the traditional PBAT29. c Comparison of the CpG coverage per sequencing depth among iscCOOL-seq, sci-MET and snmC-seq. d Boxplot of the DNA methylation levels (WCG) in individual oocytes. e Boxplot of the chromatin accessibility (GCH) in individual oocytes. f Chromatin accessibility around TSS (±1？kb) in aggregated single oocytes and individual oocytes. Shading denotes for the 25th and 75th percentile of chromatin accessibility among the single oocytes. P-values were defined by the two-tailed Student’s t-tes